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pcxle hoct3  (Addgene inc)


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    Structured Review

    Addgene inc pcxle hoct3
    Pcxle Hoct3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcxle+hoct3+4+shp53/pCXLE-hOCT3%2F4-shp53-F+(Plasmid+%2327077)/pm42543077-42-3-7
    Average 96 stars, based on 470 article reviews
    pcxle hoct3 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Reprogramming Patient-Derived urine cells into iPSCs for Anti-GAD65 autoimmune encephalitis research.
    Article Snippet: .. Briefly, 1 × 106 urine cells were electroporated with reprogramming factor (OCT4, SOX2, NANOG, KLF4, LIN28 and L-MYC) using plasmids pCXLE-hOCT3/4-shp53 (Addgene #27077), pCXLE-hSK (Addgene #27078), and pCXLE-hUL (Addgene #27080) with Neon Transfection System (Thermo Fisher Scientific) and 2 μg of each plasmid at 1200 V, 20 ms, 2 pulses. ..

    Article Title: CPAP promotes timely cilium disassembly to maintain neural progenitor pool
    Article Snippet: .. Skin fibroblasts were transfected with pCXLE‐hOct3/4‐shp53, pCXLE‐hSox2‐hKlf4, pCXLE‐hLin28‐hL‐Myc (Addgene, Cambridge, USA) using the Neon Transfection System according to the manufacturer's protocol. .. After 5 days, the medium was replaced with DMEM/F12 medium supplemented with Glutamax, 20% knockout serum replacer, 1% NAA, 0.1 mmol/l β‐mercaptoethanol (Life Technologies), and 50 ng/ml bFGF (PeproTech, Hamburg, Germany).

    Plasmid Preparation:

    Article Title: Reprogramming Patient-Derived urine cells into iPSCs for Anti-GAD65 autoimmune encephalitis research.
    Article Snippet: .. Briefly, 1 × 106 urine cells were electroporated with reprogramming factor (OCT4, SOX2, NANOG, KLF4, LIN28 and L-MYC) using plasmids pCXLE-hOCT3/4-shp53 (Addgene #27077), pCXLE-hSK (Addgene #27078), and pCXLE-hUL (Addgene #27080) with Neon Transfection System (Thermo Fisher Scientific) and 2 μg of each plasmid at 1200 V, 20 ms, 2 pulses. ..



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